Towards an understanding of dynamic genetic regulation at a global scale in bacteria: a Systems biology approach [ 2007 - 2011 ]

Research Grant

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Researchers: A/Pr Peter Lewis (Principal investigator)

Brief description To achieve major breakthroughs in the understanding of the regulation of gene transcription in bacteria at a global scale. High throughput technologies (living cell arrays, tiling DNA microarrays, multidimensional liquid chromatography proteomics and quantitative metabolomics) will be developed in conjunction with new computational modelling concepts to facilitate the understanding of biological complexity. The iterative process of simulations and model driven targeted experiments will generate novel hypotheses about the mechanistic nature of dynamic cellular responses, unravel emerging systems properties, and ultimately provide an efficient roadmap to tackle novel, pathogenic organisms.The role of my laboratory in this project is to perform subcellular localisation of transcription factors, establish standard operating procedures (with other groups) for growth, imaging and image analysis, precisely quantify 6 transcription factors under 3 standardised growth conditions for incorporation into metabolic models, and perform some analysis of transcription complexes.

Funding Amount $AUD 486,000.00

Funding Scheme NHMRC Strategic Awards

Notes Aust/EU Collaborative Research Grant

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