This beamline is dedicated to the study of microscopic samples with a wavelength-dependent spatial resolution of between 3 and 8 microns. The beamline is equipped with a Bruker Hyperion 2000 microscope with Vertex V80v FTIR (Fourier Transform Infrared) spectrometer. The following modes of data collection are available with the microscope:
* Grazing Incidence
* Micro Attenuated Total Reflectance (ATR)
In each of the above modes, a motorised stage allows the point-by-point collection of spatially resolved “spectral maps”.
Data from the IR microscope is routinely saved in either of two Bruker Opus file formats. Single spectra are saved as Opus Spectrum files. 2D IR map files are saved as OpusMultiple files. Opus Multiple files contain all spectral and x-y spatial information for a two dimensional grid map, plus related captured video images.
Opus spectrum files can be read by Bruker Opus software, CAMO The Unscrambler v 9.8 and by Thermo Grams /AI which are available at the beamline for off-line data analysis.
Opus Multiple files can be read by Bruker Opus 3D software and by CytoSpec v1.3 which are available at the beamline for off-line data analysis.
Bruker spectrum files can be batch converted into the following file formats:
* X-Y table (data point table – Excel compatible)
* SPC format (Thermo Grams AI compatible)
For assistance interpreting the data in the first instance please contact the authors of the relevant collection, or if the author is unavailable, one of the instrument scientsts (see e-mail link on this page).