Dataset

L-amino acids promote calcitonin release via a calcium-sensing receptor: Gq/11-mediated pathway in human C-cells: supplemental figures

The University of Sydney
Arthur Conigrave (Principal investigator)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://hdl.handle.net/2123/20259&rft.title=L-amino acids promote calcitonin release via a calcium-sensing receptor: Gq/11-mediated pathway in human C-cells: supplemental figures&rft.identifier= http://hdl.handle.net/2123/20259&rft.publisher=The University of Sydney&rft.description=Human calcitonin release is promoted by elevated extracellular Ca2+ (Ca2+o) concentration acting, at least in part,via the calcium-sensing receptor (CaSR). The CaSR is positively modulated by L-amino acids including the aromaticamino acids L-Phe and L-Trp. To investigate the effect of L-amino acids on human calcitonin secretion we selectedthyroid TT cells and exposed them to various Ca2+o concentrations in the absence or presence of L-Phe, plasma-likemixtures of L-amino acids, or the clinically effective positive modulator (calcimimetic), cinacalcet. In the presence ofL-Phe or plasma-like mixtures of amino acids, TT cells exhibited enhanced Ca2+o sensitivity in assays of calcitoninrelease and intracellular Ca2+ (Ca2+i) mobilization. Furthermore, the effect of elevated Ca2+o and L-Phe oncalcitonin release was markedly suppressed by the calcilytic NPS-2143. These effects were dependent uponCaSR-mediated activation of Gq/11 as revealed by the specific inhibitor YM-254890. The findings support thehypothesis that calcitonin release is stimulated by increases in plasma L-amino acid levels as well as elevatedextracellular Ca2+. They also demonstrate that stimulated calcitonin release as well as basal levels of calcitoninsecretion are mediated by a CaSR:Gq/11 signaling mechanism. This dataset comprises of 4 files; two supplemental figures, methods for supplemental figures document, and supplemental figure legends.&rft.creator=Arthur Conigrave&rft.date=2019&rft_rights= PDDL: Public Domain Dedication and License 1.0 https://opendatacommons.org/licenses/pddl/1.0/index.html&rft_subject=Medical Biochemistry and Metabolomics&rft_subject=Medical and Health Sciences&rft_subject=Pharmacology and Pharmaceutical Sciences&rft.type=dataset&rft.language=English

Licence & Rights:

Other view details
Unknown

PDDL: Public Domain Dedication and License 1.0
https://opendatacommons.org/licenses/pddl/1.0/index.html

Access:

Other view details

Brief description

Human calcitonin release is promoted by elevated extracellular Ca2+ (Ca2+o) concentration acting, at least in part,
via the calcium-sensing receptor (CaSR). The CaSR is positively modulated by L-amino acids including the aromatic
amino acids L-Phe and L-Trp. To investigate the effect of L-amino acids on human calcitonin secretion we selected
thyroid TT cells and exposed them to various Ca2+o concentrations in the absence or presence of L-Phe, plasma-like
mixtures of L-amino acids, or the clinically effective positive modulator (calcimimetic), cinacalcet. In the presence of
L-Phe or plasma-like mixtures of amino acids, TT cells exhibited enhanced Ca2+o sensitivity in assays of calcitonin
release and intracellular Ca2+ (Ca2+i) mobilization. Furthermore, the effect of elevated Ca2+o and L-Phe on
calcitonin release was markedly suppressed by the calcilytic NPS-2143. These effects were dependent upon
CaSR-mediated activation of Gq/11 as revealed by the specific inhibitor YM-254890. The findings support the
hypothesis that calcitonin release is stimulated by increases in plasma L-amino acid levels as well as elevated
extracellular Ca2+. They also demonstrate that stimulated calcitonin release as well as basal levels of calcitonin
secretion are mediated by a CaSR:Gq/11 signaling mechanism.

This dataset comprises of 4 files; two supplemental figures, methods for supplemental figures document, and supplemental figure legends.

Click to explore relationships graph
Subjects

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Identifiers