Dataset
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://www.marlin.csiro.au/geonetwork/srv/eng/search?uuid=0a0cb241-a135-4a13-8157-09dc5afc3192&rft.title=Storm Bay, March 2011, HPLC Pigment data&rft.identifier=http://www.marlin.csiro.au/geonetwork/srv/eng/search?uuid=0a0cb241-a135-4a13-8157-09dc5afc3192&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data generated from samples collected on the CMAR vessel Explorer in Storm Bay, Tasmania, between 11 and 16 March 2011. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.Water samples were taken on-board the Explorer and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere. Absorption and Pigment data was received 31/05/2016 (xls only) and archived c/o O&A Information and Data Centre. The data was archived in the OceanColourData area under StormBay_Derwent_Huon and linked to this record as a zip file.&rft.creator=Anonymous&rft.date=2016&rft.coverage=northlimit=-42.061; southlimit=-43.917; westlimit=147.116; eastLimit=148.016; projection=GDA94&rft.coverage=northlimit=-42.061; southlimit=-43.917; westlimit=147.116; eastLimit=148.016; projection=GDA94&rft_rights=Attribution 4.0 http://creativecommons.org/licenses/by/4.0/&rft_subject=Oceans&rft_subject=Pigments&rft_subject=Earth Science&rft_subject=Oceans&rft_subject=Ocean Chemistry&rft_subject=Chlorophyll&rft_subject=Suspended Solids&rft_subject=Ocean Color&rft_subject=Ocean Optics&rft_subject=Wf O Biogeochemistry&rft_subject=Remote Sensing 2008-2011&rft_subject=Coastal Waters (Australia) | Tasmania Coast East and Southeast, Tas&rft_subject=Ct Ds (Conductivity-Temperature-Depth Profilers)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Fluorometers&rft_subject=Filtration Systems&rft_subject=Hplc (High Performance Liquid Chromatography)&rft_subject=Time Series: Storm Bay - All Data&rft.type=dataset&rft.language=English Access the data

Licence & Rights:

Open Licence view details

Access:

Open

Brief description

This record contains data generated from samples collected on the CMAR vessel Explorer in Storm Bay, Tasmania, between 11 and 16 March 2011. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.

Lineage

Water samples were taken on-board the Explorer and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube.
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.
Absorption and Pigment data was received 31/05/2016 (xls only) and archived c/o O&A Information and Data Centre. The data was archived in the OceanColourData area under StormBay_Derwent_Huon and linked to this record as a zip file.

Notes

Lesley Clementson (O&A)
Christel Hassler (O&A)
Arnold Dekker (L&W)
Click to explore relationships graph

148.016,-42.061 148.016,-43.917 147.116,-43.917 147.116,-42.061 148.016,-42.061

147.566,-42.989

text: northlimit=-42.061; southlimit=-43.917; westlimit=147.116; eastLimit=148.016; projection=GDA94

Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))

uri : http://aesop.csiro.au/

Download LC_IMOS_SRS_BioOptical_Storm_Bay_March_2011_pigment.zip (.xls only) (BioOptical Storm Bay March 2011 Absorption data)

uri : http://www.cmar.csiro.au/datacentre/process/data_files/OceanColourData/StormBay_Derwent_Huon/LC_IMOS_SRS_BioOptical_Storm_Bay_March_2011_absorption.zip

Data also available via IMOS and AODN portal (IMOS and AODN portal)

uri : http://imos.aodn.org.au/webportal/

Download LC_IMOS_SRS_BioOptical_Storm_Bay_March_2011_pigment.zip (.xls only) (BioOptical Storm Bay March 2011 Pigment data)

uri : http://www.cmar.csiro.au/datacentre/process/data_files/OceanColourData/StormBay_Derwent_Huon/LC_IMOS_SRS_BioOptical_Storm_Bay_March_2011_pigments.zip

Identifiers